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ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. Which of the following CD markers is NOT present on a normal mature T cell?

A) CD5
B) CD7
C) CD20
D) CD3

2. Which of the following could reduce the risk of a patient becoming infected with a hospital acquired infection?

A) Proper handwashing procedure
B) Chemotherapy
C) Antibiotic therapy
D) Pre-surgical testing

3. Rule-out is a process by which antibodies are identified as being unlikely in a given sample because of the absence of an expected antigen-antibody reaction. In other words, the absence of a reaction is noted with a cell that is positive for the corresponding antigen.
Although rule-out procedures may vary somewhat from institution to institution, the following general principles apply:
Non-reactive cells are selected for rule-out. To be classified as non-reactive, a cell must NOT have reacted at any phase of testing in a given panel or screen.
Using the logic that if the rule-out cell is positive for a given antigen, it should have reacted with the corresponding antibody, you can rule-out antibodies that correspond to antigen positive cells.
To increase the probability that rule-out will not mistakenly eliminate a weakly-reacting antibody that exhibits dosage*, use only cells that are homozygous for the corresponding antigen for those systems that generally show dosage. Generally these include: C, c, E, e, Fya, Fyb, Jka, Jkb, M, N, S, and s.
In this case, it is only possible to rule out on screening cell 2 since it demonstrates a negative reaction with the patient serum. Anti-C cannot be ruled out since the C antigen is heterozygous on screening cell 2 with c.
Anti-Fya cannot be ruled out since this antigen is not present on screening cell 2. Anti-M and anti-Jka can be ruled out since the antigens are homozyous while demonstrating a negative reaction on screening cell 2.
Rule-out, while very useful, can lead to error. Ruling out an antibody should be combined with other supporting data to increase confidence in the solution; the more data collected, the higher the probability that the final solution is correct.
*Dosage means that there are two "doses" of the same antigen present on the red cells . Antibodies that exhibit dosage react more strongly with homozygous cells (e.g., Jka Jka) than with heterozygous cells (e.g., Jka Jkb) .
Based on the phenotype of the RBC screening cells, and patient results shown on the right, which of the following antibodies CANNOT be ruled out?

A) Anti-Jka
B) Anti-Fya
C) Anti-M
D) Anti-C

4. Myoglobin rises typically within 2-4 hours after a myocardial infarction and stay elevated for days, with a peak at the 6-9-hour mark post-MI. After myoglobin, troponin is the next fastest to rise afer a mycardial infarction and can rise within 3-4 hours with a peak around 12 hours post-MI. LD (8-12 hours, 24-48 hour peak) and CK (4-6 hours with peak at 24-36 hours) will also rise after MI, but are not as fast to rise as myoglobin and troponin.
Chemistry
A patient is admitted to the hospital with acute chest pain, but which of the following cardiac markers will be elevated FIRST if the patient had an MI?

A) LD
B) CK
C) Troponin
D) Myoglobin

5. Micro - Matching
1. Upper left image
Multi-celled, rough walled macroconidia with a tapered terminal cell
2. Upper right image
Dense aggregates of echinulate, brown-black conidia
3. Lower left image
Loose clusters of elliptical conidia arranged in a "diphtheroid" pattern
4. Lower right image
Chains of large, lemon-shaped annelloconidia

A) Microsporum canis
B) Aspergillus niger
C) Scopulariopsis species
D) Acremonium species

Solutions: